G-Biosciences offers G-Trap™ Immobilized Amino Acids resin in a pre-packed column format. Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking the amino or sulfhydryl group of the amino acid to the preactivated resin.
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Features:
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Amino acid coupled to 6% cross-linked Agarose
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Coupling is covalent through linkage of amino or sulfhydryl group of the amino acid to the pre-activated agarose.
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Shipped as hydrated resin in 20% ethanol
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Available in 1ml and 5ml sizes in a prepacked column
Void volume: ~0.3 ml for 1 ml column and ~1.5 ml for 5 ml column
Sample Load volume: 0.03 ml- 0.3 ml for 1 ml column and 0.75-1.5 ml for 5 ml column
Recommended Flow rate: 1 ml/min for 1 m column and 3 ml/min for 5 ml column
Chemical stability: All commonly used buffers, 8M urea, 6M guanidine hydrochloride and all non-ionic detergents. Lower alcohols such as methanol, ethanol and propanol can be added to buffers at lower than 25 V/V %
pH stability: 2 to 13
Storage: 20 % ethanol at 4°C to 30°C
G-Trap™ Amino Acids Column, 1 ml
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
G-Trap™ Amino Acids Column, 5 ml
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- Immobilized Amino Acids are used for purification of amino acid binding proteins.
